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Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through <t>IVIS</t> imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.
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Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through <t>IVIS</t> imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.
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Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through <t>IVIS</t> imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.
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Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through <t>IVIS</t> imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.
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Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through <t>IVIS</t> imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.
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Image Search Results


Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through IVIS imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.

Journal: Cell

Article Title: Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery.

doi: 10.1016/j.cell.2023.03.033

Figure Lengend Snippet: Figure 3. Myogenic progenitors in dystrophic mice are transduced by lentiviruses pseudotyped with Myomaker and Myomerger (A) Top displays experimental design for analysis of muscle progenitors. Middle shows representative FACS plots and quantification for a7-Integrin+ myogenic cells (y axis) and tdTomato+ cells (x axis) from mdx4cv; RosatdTom muscle that received Bald-LV-Cre or Mymk+Mymg-LV-Cre. Bottom shows representative FACS plots and quantification for tdTomato+ non-myogenic interstitial cells. (B) Top is the experimental design using lentivirus encoding for luciferase that was pseudotyped with Bald, VSV-G, or Myomaker and Myomerger. After car- diotoxin injury, lentiviruses were injected intramuscularly and bioluminesence measured through IVIS imaging multiple time points after viral delivery. Repre- sentative pseudocolored images show a progressive increase in bioluminescence in the muscles treated with Myomaker and Myomerger-pseudotyped virus. Bottom is quantification of bioluminescence for muscles transduced with lentivirus containing Myomaker and Myomerger. (C) Mdx4cv; RosatdTom mice received an intramuscular injection of Bald-LV-Cre or Mymk+Mymg-LV-Cre, then 2 weeks later some mice were injured with car- diotoxin. Representative images for tdTomato+ myofibers are shown to the right. Nuclei were stained with DAPI. Scale bar, 100 mm. Bottom left shows quan- tification of the percentage of tdTomato+ myofibers. Data are presented as mean ± standard deviation; data in (A, B) combined from at least two independent LV preparations; data in (C) is from one LV preparation. Statistical tests used were (A) unpaired t test with Welch’s correction; (B) Friedman test with Dunn’s correction for multiple comparisons; (C) two-way ANOVA with Tukey’s correction for multiple comparisons; **p < 0.01, ****p < 0.0001.

Article Snippet: The bioluminescence images were acquired 7 min after D-luciferin injection with a rate of one image per 1min for 30 min using the IVIS spectrum CT in vivo imaging system (PerkinElmer, Cat#128201).

Techniques: Luciferase, Injection, Imaging, Muscles, Virus, Transduction, Staining, Standard Deviation